7 results

Description and evaluation of experimental models for uterine transplantation in pigs

Emerson de Oliveira, Kelly Alessandra da Silva Tavares, Mariano Tamura Vieira Gomes, Alcides Augusto Salzedas-Netto, Marair Gracio Ferreira Sartori, Rodrigo Aquino Castro, [...]

DOI: 10.1590/S1679-45082017AO4066

ABSTRACT Objective: To evaluate the technique of uterine transplantation and the use of drugs used in the process of immunosuppression. Methods: We included 12 sows, and immunosuppression was performed with minimal doses of cyclosporine, and cross-match was done to exclude the possibility of blood incompatibility. Hysterectomy was performed in the donor under general anesthesia, with the removal of the aorta and inferior vena cava in monobloc, and anastomosis of these vessels was made in the recipient. Results: Six experiments were […]

Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode

Marcelo Vivolo Aun, Beatriz Mangueira Saraiva-Romanholo, Francine Maria de Almeida, Thayse Regina Brüggemann, Jorge Kalil, Milton de Arruda Martins, [...]

DOI: 10.1590/S1679-45082015AO3389

ABSTRACTObjective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization.Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or […]

Histological analysis of the repair of dural lesions with silicone mesh in rats subjected to experimental lesions

Fernando William Figueiredo da Rosa, Pedro Henrique Isoldi Pohl, Ana Maria Amaral Antônio Mader, Carla Peluso de Paiva, Aline Amaro dos Santos, Bianca Bianco, [...]

DOI: 10.1590/S1679-45082015AO3378

ABSTRACTObjective To evaluate inflammatory reaction, fibrosis and neovascularization in dural repairs in Wistar rats using four techniques: simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture.Methods Thirty Wistar rats were randomized in five groups: the first was the control group, submitted to dural tear only. The others underwent durotomy and simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Animals were euthanized and the spine was submitted to histological evaluation with a score system […]

Superoxide overproduction and kidney fibrosis: a new animal model

Nadia Karina Guimarães-Souza, Liliya Marsovna Yamaleyeva, Baisong Lu, Ana Claudia Mallet de Souza Ramos, Colin Edward Bishop, Karl Erik Andersson

DOI: 10.1590/S1679-45082015AO3179

ObjectiveTo establish whether the mutation in the Immp2L gene induces renal fibrosis and whether aging exacerbates renal morphology in mice.MethodsFemale mutant mice with mutation in the inner mitochondrial membrane peptidase 2-like protein at 3 and 18 months of age were used. Renal fibrosis was analyzed using classic fibrosis score, Masson’s trichrome staining, and analysis of profibrotic markers using real time polymerase chain reaction (superoxide dismutase 1, metalloproteinase-9, erythropoietin, transforming growth factor beta), and immunostaining (fibroblasts and Type IV collagen). Oxidative […]

Maternal behavior in basic science: translational research and clinical applicability

Gabriel Natan Pires, Sergio Tufik, Márcia Giovenardi, Monica Levy Andersen

DOI: 10.1590/S1679-45082013000200021

Clinical aspects of the mother-infant relationship and related issues are well studied and very relevant to medical practice. Nevertheless, some approaches in this context cannot plausibly be investigated in humans due to their ethical implications and to the potential harm to the mother’s and child’s health. Studies on maternal behavior in animals have evident importance to some clinical fields, such as psychiatry and psychology, particularly considering topics, including mother-infant relationship, postpartum depression, cognitive and behavioral development of children, and associated […]

Standardization of an experimental model suitable for studies on the effect of exercise on arthritis

Raquel Pinheiro Gomes, Elisangela Bressan, Tatiane Morgana da Silva, Monique da Silva Gevaerd, Carlos Rogério Tonussi, Susana Cristina Domenech

DOI: 10.1590/S1679-45082013000100014

Objective:To standardize an experimental model of chronic monoarthritis induced by complete Freund’s adjuvant appropriate for the analysis of the effect of walking on nociception and on joint edema.METHODS:The following factors were evaluated as to monoarthritis induction: route and site of administration, number and interval of inoculations, Mycobacterium species, and animal gender. Wistar male and female rats (200 to 250g) received two injections of complete Freund’s adjuvant containing Mycobacterium tuberculosis (1.0mg/mL; 50µL) or Mycobacterium butyricum (0.5mg/mL; 50µL) intra-articularly in the tibiotarsal […]

Magnetohyperthermia for treatment of gliomas: experimental and clinical studies

André César da Silva, Tiago Ribeiro Oliveira, Javier Bustamante Mamani, Suzana Mária Fleury Malheiros, Lorena Favaro Pavon, Tatiana Taís Sibov, [...]

DOI: 10.1590/s1679-45082010rw1757

ABSTRACTGliomas comprise a group of heterogeneous primary tumors of the central nervous system that originate from glial cells. Malignant gliomas account for the majority of primary malignant CNS tumors and are associated with high morbidity and mortality. Glioblastoma is the most frequent malignant glioma, and despite recent advances in diagnosis and new treatment options, its prognosis remains dismal. New opportunities for the development of effective therapies for malignant gliomas are urgently needed. Magnetohyperthermia consists of heat generation in the region […]