6 results

Human papillomavirus infection and cervical cancer precursor lesions in women living by Amazon rivers: investigation of relations with markers of oxidative stress

Bruna Emanuelle Sanches Borges ORCID logo , Elza Baia de Brito ORCID logo , Hellen Thais Fuzii ORCID logo , Cláudia Simone Baltazar ORCID logo , Aline Barreto Sá ORCID logo , Camile Irene Mota da Silva ORCID logo , [...]

DOI: 10.1590/S1679-45082018AO4190

ABSTRACT Objective To investigate the relation between oxidative stress markers, human papillomavirus infection and cervical cancer precursor lesions. Methods The study comprised women aged 14 to 60 years living in communities located by Amazon rivers in the state of Pará (Itaituba, Limoeiro do Ajuru and Bragança, 126, 68 and 43 women respectively). Papanicolau smears and polymerase chain reaction tests for human papillomavirus DNA detection were performed. Blood samples were collected to test malondialdehyde, total and oxidized glutathione levels. Results Malondialdehyde, […]

Assessment of oxidative stress in saliva of children with dental erosion

Caleb Shitsuka, Flávia Kazue Ibuki, Fernando Neves Nogueira, Fausto Medeiros Mendes, Marcelo Bönecker

DOI: 10.1590/S1679-45082018AO4203

ABSTRACT Objective To evaluate oxidative stress in saliva of children with dental erosion as compared to children with no erosion. Methods One single examiner, trained and prepared to make diagnosis of dental erosion according to the Basic Erosive Wear Examination index, selected 40 children aged 4 to 6 years, who attended a pediatric dentistry prevention clinic. Two groups were formed – one comprising children with dental erosion (n=22), and another with no dental erosion (n=18). The quantity of dental biofilm […]

Superoxide overproduction and kidney fibrosis: a new animal model

Nadia Karina Guimarães-Souza, Liliya Marsovna Yamaleyeva, Baisong Lu, Ana Claudia Mallet de Souza Ramos, Colin Edward Bishop, Karl Erik Andersson

DOI: 10.1590/S1679-45082015AO3179

ObjectiveTo establish whether the mutation in the Immp2L gene induces renal fibrosis and whether aging exacerbates renal morphology in mice.MethodsFemale mutant mice with mutation in the inner mitochondrial membrane peptidase 2-like protein at 3 and 18 months of age were used. Renal fibrosis was analyzed using classic fibrosis score, Masson’s trichrome staining, and analysis of profibrotic markers using real time polymerase chain reaction (superoxide dismutase 1, metalloproteinase-9, erythropoietin, transforming growth factor beta), and immunostaining (fibroblasts and Type IV collagen). Oxidative […]

Oxidative stress and plasma lipoproteins in cancer patients

Fernanda Maria Machado Maia, Emanuelly Barbosa Santos, Germana Elias Reis

DOI: 10.1590/S1679-45082014RC3110

ObjectiveTo evaluate the relation between oxidative stress and lipid profile in patients with different types of cancer.MethodsThis was an observational cross-sectional. A total of 58 subjects were evaluated, 33 males, divided into two groups of 29 patients each: Group 1, patients with cancer of the digestive tract and accessory organs; Group 2 patients with other types of cancers, all admitted to a public hospital. The plasma levels (lipoproteins and total cholesterol, HDL, and triglycerides, for example) were analyzed by enzymatic […]

Evaluation of the concentration of malondialdehyde and nitrite in patients with sickle cell anemia treated or not with hydroxyurea

Darcielle Bruna Dias Elias, Rivelilson Mendes de Freitas, Romélia Pinheiro Gonçalves, Hemerson Yuri Ferreira Magalhães, Jacqueline Holanda de Sousa, Silvia Maria Meira Magalhães

DOI: 10.1590/S1679-45082010AO1731

ABSTRACT Objective: To determine the serum levels of malondialdehyde and nitrite in patients with sickle cell anemia treated or not with hydroxyurea in outpatient’s setting. Methods: Of the 65 patients with sickle cell anemia selected for the study, 51 were not treated with hydroxyurea (Group 1), 14 made chronic use of hydroxyurea (Group 2) and 20 individuals had no hemoglobinopathies (Control Group). Results: The Control Group had a lower and more homogeneous concentration of malondialdehyde levels as compared to the […]

Pathogenesis of pulmonary emphysema – cellular and molecular events

Antonio Di Petta

DOI: 10.1590/s1679-45082010rb1480

ABSTRACTPulmonary emphysema is a chronic obstructive disease, resulting from important alterations in the whole distal structure of terminal bronchioles, either by enlargement of air spaces or by destruction of the alveolar wall, leading to loss of respiratory surface, decreased elastic recoil and lung hyperinflation. For many years, the hypothesis of protease-antiprotease unbalance prevailed as the central theme in the pathogenesis of pulmonary emphysema. According to this hypothesis, the release of active proteolytic enzymes, produced mainly by neutrophils and macrophages, degrades […]