Highlights ■ Colorectal carcinoma originates from within cancer stem cells of the large intestine. ■ Tissue levels of ALDH1-2 and LGR5 proteins were  associated with greater tumor aggressiveness. ■ Discovery of signaling pathways can improve cancer treatments that control cancer stem cells. ABSTRACT Objective: To investigate the association of CD44, CD133, ALDH1-2, and LGR5 expression with clinicopathological tumor variables. Methods: Twenty-eight adult patients with colorectal carcinoma who underwent curative surgery were included in the study. Paraffin-embedded samples were subjected to […]

OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the […]