All publications of “Suzana Maria Fleury Malheiros”

4 results

Glioblastoma: approach to treat elderly patients

Luciola de Barros Pontes, Theodora Karnakis, Suzana Maria Fleury Malheiros, Eduardo Weltman, Reynaldo André Brandt, Rafael Aliosha Kaliks Guendelmann

DOI: 10.1590/S1679-45082012000400021

Treating elderly cancer patients is a challenge for oncologists, especially considering the several therapeutic modalities in glioblastoma. Extensive tumor resection offers the best chance of local control. Adequate radiotherapy should always be given to elderly patients if they have undergone gross total resection and have maintained a good performance status. Rather than being ruled out, chemotherapy should be considered, and temozolomide is the chosen drug. A comprehensive geriatric assessment is a valuable tool to help guiding treatment decisions in elderly […]

Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

Lorena Favaro Pavon, Luciana Cavalheiro Marti, Tatiana Tais Sibov, Liza Aya Mabuchi Miyaki, Suzana Maria Fleury Malheiros, Javier Bustamante Mamani, [...]

DOI: 10.1590/S1679-45082012000200013

Objective:To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set.METHODS:Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the […]

Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy

André César da Silva, Francisco Romero Cabral, Javier Bustamante Mamani, Jackeline Moraes Malheiros, Roberson Saraiva Polli, Alberto Tannus, [...]

DOI: 10.1590/S1679-45082012000100004

Objective:The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy.METHODS:Young male Wistar rats weighing between 250 and 300 g were used for the C6 model. After the rates were anesthetized (55 mg/kg ketamine and 11 mg/kg xylazine), C6 lineage tumorigenic cells suspended in culture medium (10(5) cells in 10 µl) were stereotaxically […]

Magnetohyperthermia for treatment of gliomas: experimental and clinical studies

André César da Silva, Tiago Ribeiro Oliveira, Javier Bustamante Mamani, Suzana Mária Fleury Malheiros, Lorena Favaro Pavon, Tatiana Taís Sibov, [...]

DOI: 10.1590/s1679-45082010rw1757

ABSTRACTGliomas comprise a group of heterogeneous primary tumors of the central nervous system that originate from glial cells. Malignant gliomas account for the majority of primary malignant CNS tumors and are associated with high morbidity and mortality. Glioblastoma is the most frequent malignant glioma, and despite recent advances in diagnosis and new treatment options, its prognosis remains dismal. New opportunities for the development of effective therapies for malignant gliomas are urgently needed. Magnetohyperthermia consists of heat generation in the region […]