2 results

Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

Lorena Favaro Pavon, Luciana Cavalheiro Marti, Tatiana Tais Sibov, Liza Aya Mabuchi Miyaki, Suzana Maria Fleury Malheiros, Javier Bustamante Mamani, [...]

DOI: 10.1590/S1679-45082012000200013

Objective:To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set.METHODS:Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the […]

Isolation of human umbilical cord blood-derived osteoprogenitor cells: a promising candidate for cell-based therapy for bone repair

Igor Iuco Castro-Silva, Letícia de Oliveira Castro, Janaína José dos Santos Machado, Maria Helena Alves Nicola, José Mauro Granjeiro

DOI: 10.1590/s1679-45082011ao2196

ABSTRACTObjective:The aim of this study was to evaluate the osteogenic potential of human umbilical cord blood-derived osteoprogenitor cells and to prove its applicability as a promising candidate for cell-based therapeutics for bone repair.Methods:Primary cultures of human umbilical blood cord adherent cells were expanded in vitro until passage 2 and seeded for osteodifferentiation study. Morphological (light microscopy), cytochemical (Von Kossa’s method), and functional analyses (calcium level, alkaline phosphatase activity, and total protein content in cell culture) were carried out 7, 14, […]