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Isolation of human umbilical cord blood-derived osteoprogenitor cells: a promising candidate for cell-based therapy for bone repair

Igor Iuco Castro-Silva, Letícia de Oliveira Castro, Janaína José dos Santos Machado, Maria Helena Alves Nicola, José Mauro Granjeiro

DOI: 10.1590/s1679-45082011ao2196

ABSTRACTObjective:The aim of this study was to evaluate the osteogenic potential of human umbilical cord blood-derived osteoprogenitor cells and to prove its applicability as a promising candidate for cell-based therapeutics for bone repair.Methods:Primary cultures of human umbilical blood cord adherent cells were expanded in vitro until passage 2 and seeded for osteodifferentiation study. Morphological (light microscopy), cytochemical (Von Kossa’s method), and functional analyses (calcium level, alkaline phosphatase activity, and total protein content in cell culture) were carried out 7, 14, […]