3 results

Study of internalization and viability of multimodal nanoparticles for labeling of human umbilical cord mesenchymal stem cells

Liza Aya Mabuchi Miyaki, Tatiana Tais Sibov, Lorena Favaro Pavon, Javier Bustamante Mamani, Lionel Fernel Gamarra

DOI: 10.1590/S1679-45082012000200012

Objective:To analyze multimodal magnetic nanoparticles-Rhodamine B in culture media for cell labeling, and to establish a study of multimodal magnetic nanoparticles-Rhodamine B detection at labeled cells evaluating they viability at concentrations of 10µg Fe/mL and 100µg Fe/mL.METHODS:We performed the analysis of stability of multimodal magnetic nanoparticles-Rhodamine B in different culture media; the mesenchymal stem cells labeling with multimodal magnetic nanoparticles-Rhodamine B; the intracellular detection of multimodal magnetic nanoparticles-Rhodamine B in mesenchymal stem cells, and assessment of the viability of labeled […]

Isolation of human umbilical cord blood-derived osteoprogenitor cells: a promising candidate for cell-based therapy for bone repair

Igor Iuco Castro-Silva, Letícia de Oliveira Castro, Janaína José dos Santos Machado, Maria Helena Alves Nicola, José Mauro Granjeiro

DOI: 10.1590/s1679-45082011ao2196

ABSTRACTObjective:The aim of this study was to evaluate the osteogenic potential of human umbilical cord blood-derived osteoprogenitor cells and to prove its applicability as a promising candidate for cell-based therapeutics for bone repair.Methods:Primary cultures of human umbilical blood cord adherent cells were expanded in vitro until passage 2 and seeded for osteodifferentiation study. Morphological (light microscopy), cytochemical (Von Kossa’s method), and functional analyses (calcium level, alkaline phosphatase activity, and total protein content in cell culture) were carried out 7, 14, […]

Does the time between collecting and processing umbilical cord blood samples affect the quality of the sample?

Ricardo Barini, Ubirajara Costa Ferraz, Gregório Lorenzo Acácio, Isabela Nelly Machado

DOI: 10.1590/S1679-45082011GS1809

ABSTRACT Objective: To assess the association between the time from umbilical cord blood collection until processing and the quality of the sample. Methods: Umbilical cord blood samples collected during the third stage of labor were placed in temperature-controlled boxes for the transport of biological material and sent to an umbilical cord blood bank, where the number of nucleated cells, viable cells and CD34+ cells were counted, and samples were additionally tested for contamination at the following time intervals: up to […]