All publications of “Gregório Lorenzo Acácio”

2 results

Does the time between collecting and processing umbilical cord blood samples affect the quality of the sample?

Ricardo Barini, Ubirajara Costa Ferraz, Gregório Lorenzo Acácio, Isabela Nelly Machado

DOI: 10.1590/S1679-45082011GS1809

ABSTRACT Objective: To assess the association between the time from umbilical cord blood collection until processing and the quality of the sample. Methods: Umbilical cord blood samples collected during the third stage of labor were placed in temperature-controlled boxes for the transport of biological material and sent to an umbilical cord blood bank, where the number of nucleated cells, viable cells and CD34+ cells were counted, and samples were additionally tested for contamination at the following time intervals: up to […]

Modification of the gasless fetoscopy technique for the treatment of large myelomeningocele: a study in sheep

Denise Araujo Lapa Pedreira, Gregório Lorenzo Acácio, Rogério Chaccur Abou-Jamra, Rita de Cássia Sanches Oliveira, Elia Garcia Caldini, Paulo Hilário Nascimento Saldiva

DOI: 10.1590/s1679-45082010ao1440

ABSTRACTObjective:To change the gasless fetoscopy technique in order to reduce the diameter of entry orifices in the myometrium.Methods:Seven pregnant ewes were submitted to fetoscopy for repairing a large skin defect measuring 4.0 × 3.0 cm, created in the fetal lumbar region at the gestational age of 100 days. The defect was repaired through continuous suture of the skin with approximation of borders. Gasless fetoscopy was used for performing the suture with three orifices to allow entry of the trocar into […]