All publications of “Jaques Waisberg”

4 results

The destruction complex of beta-catenin in colorectal carcinoma and colonic adenoma

Guilherme Muniz Bourroul, Hélio José Fragoso, José Walter Feitosa Gomes, Vivian Sati Oba Bourroul, Celina Tizuko Fujiyama Oshima, Thiago Simão Gomes, [...]

DOI: 10.1590/S1679-45082016AO3678

ABSTRACTObjectiveTo evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catetenin, adenomatous polyposis coli, GSK3β, axin and ubiquitin in colorectal carcinoma and colonic adenoma.Methods Tissue samples from 64 patients with colorectal carcinoma and 53 patients with colonic adenoma were analyzed. Tissue microarray blocks and slides were prepared and subjected to immunohistochemistry with polyclonal antibodies in carcinoma, adjacent non-neoplastic mucosa, and adenoma tissues. The immunoreactivity was evaluated by the percentage of positive stained cells and by the intensity […]

Glycomics expression analysis of sulfated glycosaminoglycans of human colorectal cancer tissues and non-neoplastic mucosa by electrospray ionization mass spectrometry

Ana Paula Cleto Marolla, Jaques Waisberg, Gabriela Tognini Saba, Daniel Reis Waisberg, Fernando Beani Margeotto, Maria Aparecida da Silva Pinhal

DOI: 10.1590/S1679-45082015AO3477

ABSTRACTObjective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression.Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans […]

Immunoexpression of the COX-2, p53, and caspase-3 proteins in colorectal adenoma and non-neoplastic mucosa

Renan Brito Nogueira, Andréa Rodrigues Cordovil Pires, Thélia Maria Santos Soares, Simone Rabello de Souza Rodrigues, Mariane Antonieta Menino Campos, Giovanna Canato Toloi, [...]

DOI: 10.1590/S1679-45082013000400009

Objective:To analyze the immunoexpression of the COX-2, p53, and caspase-3 proteins in colorectal adenomas and non-neoplastic mucosa.METHODS:72 individuals were subjected to colonoscopy, which provided 50 samples of adenomas and 45 samples of non-neoplastic colorectal mucosa. The tissue samples were obtained via the tissue microarray technique and subjected to immunohistochemical analysis using primary anti-p53, anti-COX-2, and anti-caspase-3 antibodies. The positivity and intensity of the immunoreaction were classified. The analyzed variables were as follows: site of the adenomas in the colon, degree […]

En bloc pancreaticoduodenectomy and right hemicolectomy to treat locally advanced right colon cancer: report of three cases

Sergio Renato Pais Costa, Sergio Henrique Couto Horta, Alexandre Cruz Henriques, Jaques Waisberg, Manlio Basílio Speranzini

DOI: 10.1590/s1679-45082010rc786

ABSTRACTAlthough colorectal tumors are fairly common surgical conditions, 5 to 12% of these tumors are locally advanced (T4 tumors) upon diagnosis. In this particular situation, the efficacy of en bloc multivisceral resection has been proven. When right-colon cancer invades the proximal duodenum or even the pancreatic head, a challenging dilemma arises due to complexity of the curative surgical procedure. Therefore, en bloc pancreaticoduodenectomy with right hemicolectomy should be performed to obtain free margins. The present study reports three cases of […]