The journal einstein (São Paulo) – e-ISSN 2317-6385 is dedicated to dissemination of high-quality scientific content that advances our understanding of human disease with the goal of improving prevention, care, diagnostics and treatment of patients globally.
To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols.
Methods:
A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria.
Results:
Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection.
Conclusion:
High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.
Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
CorreiaRP, BentoLC, BortolucciACA, AlexandreAM, VazAC, SchimidellD, PedroEC, et al. Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria. Einstein (Sao Paulo). 2016;14(3):366-73. https://doi.org/10.1590/S1679-45082016AO3641
Correia,Rodolfo Patussi; Bento,Laiz Cameirão; Bortolucci,Ana Carolina Apelle; Alexandre,Anderson Marega; Vaz,Andressa da Costa; Schimidell,Daniela; Pedro,Eduardo de Carvalho; Perin,Fabricio Simões; Nozawa,Sonia Tsukasa; Mendes,Cláudio Ernesto Albers; Barroso,Rodrigo de Souza; Bacal,Nydia Strachman. Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria. Einstein (Sao Paulo)., v. 14, n. 3, p. 366-373, Jul. 2016. https://doi.org/10.1590/S1679-45082016AO3641
Correia,R.P., Bento,L.C., Bortolucci,A.C.A., Alexandre,A.M., Vaz,A.C., Schimidell,D., Pedro,E.C., Perin,F.S., Nozawa,S.T., Mendes,C.E.A., Barroso,R.S., & Bacal,N.S. (2016). Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria. Einstein (Sao Paulo).,14(3), 366-373. https://doi.org/10.1590/S1679-45082016AO3641
Correia,Rodolfo Patussi and Bento,Laiz Cameirão and Bortolucci,Ana Carolina Apelle and Alexandre,Anderson Marega and Vaz,Andressa da Costa and Schimidell,Daniela and Pedro,Eduardo de Carvalho and Perin,Fabricio Simões and Nozawa,Sonia Tsukasa and Mendes,Cláudio Ernesto Albers and Barroso,Rodrigo de Souza and Bacal,Nydia Strachman. Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria. Einstein (Sao Paulo). [online]. 2016, vol. 14, n. 3, [cited 2025-09-27], pp.366-373. Available from: <https://journal.einstein.br/article/technical-advances-in-flow-cytometry-based-diagnosis-and-monitoring-of-paroxysmal-nocturnal-hemoglobinuria/>. ISSN 1679-4508. https://doi.org/10.1590/S1679-45082016AO3641
Figure 7
Activity-based costing. Paroxysmal nocturnal hemoglobinuria test cost reduction was associated with technical procedures. The two-color assay was taken as the starting point for comparative analysis of technical procedures in this study (two tubes four-color and single tube five-color assays)
Activity-based costing. Paroxysmal nocturnal hemoglobinuria test cost reduction was associated with technical procedures. The two-color assay was taken as the starting point for comparative analysis of technical procedures in this study (two tubes four-color and single tube five-color assays)
