All publications of “Nydia Strachman Bacal”

6 results

Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

Rodolfo Patussi Correia, Laiz Cameirão Bento, Ana Carolina Apelle Bortolucci, Anderson Marega Alexandre, Andressa da Costa Vaz, Daniela Schimidell, [...]

DOI: 10.1590/S1679-45082016AO3641

ABSTRACTObjective:To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols.Methods:A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria.Results:Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection.Conclusion:High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in […]

Correlation between flow cytometry and histologic findings: ten year experience in the investigation of lymphoproliferative diseases

Alanna Mara Pinheiro Sobreira Bezerra, Denise da Cunha Pasqualin, João Carlos de Campos Guerra, Marjorie Paris Colombini, Elvira Deolinda Rodrigues Pereira Velloso, Paulo Augusto Achucarro Silveira, [...]

DOI: 10.1590/S1679-45082011AO2027

ABSTRACT Objective: To demonstrate the advantages of correlating flow cytometry immunophenotyping with the pathology/ immunohistochemistry of lymph nodes or nodules in the diagnosis of lymphoproliferative diseases. Methods: A retrospective study was carried out of 157 biopsy or fine-needle aspiration lymph nodes/ nodule specimens taken from 142 patients, from 1999 and 2009. The specimens were simultaneously studied with fow cytometry and pathology at Hospital Israelita Albert Einstein. The specimens were prepared in hematoxylin/eosin, Giemsa, or monoclonal antibody stained slides for detecting […]

Molecular and cytogenetic abnormalities in acute myeloid leukemia: review and case studies

Elvira Deolinda Rodrigues Pereira Velloso, Carlos Henrique Ares Silveira da Motta, Juliana Braga Furtado, Nydia Strachman Bacal, Paulo Augusto Achucarro Silveira, Cynthia Bachir Moyses, [...]

DOI: 10.1590/S1679-45082011AO2041

ABSTRACT Objective: To study the frequency of mutations that may lead to a good or bad prognosis, as well as their relation with the karyotype and immunophenotype in patients with acute myeloid leukemia. Methods: Thirty samples of patients with acute myeloid leukemia were studied, in which FLT3-ITD, FLT3-TKD and NPM1 mutations were investigated. All samples were submitted to immunophenotyping and 25 to karyotyping. Results: An occurrence of 33.3% NPM1 mutation and an equal number of FLT3-ITD mutation were observed. When […]

Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies

João Carlos de Campos Guerra, Ruth Hissae Kanayama, Sonia Tsukasa Nozawa, Márcia Regina Ioshida, Irina Yoko Takiri, Robson José Lazaro, [...]

DOI: 10.1590/S1679-45082011AO1846

ABSTRACT Objective: To identify antiplatelet antibodies by flow cytometry (direct method) in patients with thrombocytopenia. Methods: Between January 1997 and March 2004 a total of 15100 patients were referred to the Centro de Hematologia de São Paulo for hematological investigation of several diagnoses (anemia, leukopenia, thrombocytopenia, coagulation abnormalities, adenomegaly, leukemia and others). Of those, 1057 were referred because of thrombocytopenia and were divided into two groups: Group Idiopathic thrombocytopenic purpura, with no identifiable cause; and Group Other thrombocytopenia, which included […]