einstein (São Paulo). 24/Apr/2019;17(2):eAO4328.
Applicability of papain solutions in immunohematology
DOI: 10.31744/einstein_journal/2019AO4328
ABSTRACT
Objective:
To compare the enzyme activity of different presentations of papain solution to validate in-house preparations.
Methods:
Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD.
Results:
In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution.
Conclusion:
Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
Keywords: Antigens; Allergy and immunology; Papain; Enzymes
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