1 results
01/Apr/2014
DOI: 10.1590/S1679-45082014AO2932
Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture. Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests. Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to […]
Keywords: Multiplex polymerase chain reaction; Real-time polymerase chain reaction; Sepsis/diagnosis