All publications of “Roberta Sitnik”

5 results

Personalized medicine and the clinical laboratory

João Renato Rebello Pinho, Roberta Sitnik, Cristóvão Luis Pitangueira Mangueira

DOI: 10.1590/s1679-45082014rw2859

Personalized medicine is the use of biomarkers, most of them molecular markers, for detection of specific genetic traits to guide various approaches for preventing and treating different conditions. The identification of several genes related to heredity, oncology and infectious diseases lead to the detection of genetic polymorphisms that are involved not only in different clinical progression of these diseases but also in variations in treatment response. Currently, it is possible to detect these polymorphisms using several methodologies: detection of single […]

SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital

Roberta Sitnik, Alexandre Rodrigues Marra, Roberta Cardoso Petroni, Ozires Pereira Santos Ramos, Marinês Dalla Valle Martino, Jacyr Pasternak, [...]

DOI: 10.1590/S1679-45082014AO2932

ObjectiveTo test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.MethodsA total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.ResultsMore positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only […]

The detection of KIT mutations in acute myeloid leukemia

Luis Eduardo Silva Machado, João Renato Rebello Pinho, Roberta Sitnik, Nair Hideko Muto, Elvira Deolinda Rodrigues Pereira Velloso, Roberta Cardoso Petroni, [...]

DOI: 10.1590/S1679-45082012000300005

Objective:This study describes a new method used in the clinical laboratory at Hospital Israelita Albert Einstein to detect mutations in exons 8 and 17 of the KIT gene in patients with acute myeloid leukemia.METHODS:Genomic DNA extraction was performed on 54 samples of peripheral blood or bone marrow from patients with acute myeloid leukemia. The extracted DNA was amplified by polymerase chain reaction and sequenced, and the fragments were analyzed.RESULTS:Within the analyzed samples, we detected four mutations in exon 8, two […]

Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes

Flavia Maziero Andreghetto, Maria Fatima Guarizo Klingbeil, Renata Machado Soares, Roberta Sitnik, Décio dos Santos Pinto Junior, Monica Beatriz Mathor, [...]

DOI: 10.1590/s1679-45082011ao2149

ABSTRACTObjective:Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas.Methods:The expression level of four microRNAs was assessed in cell lines and in primary cultures of […]

Molecular and cytogenetic abnormalities in acute myeloid leukemia: review and case studies

Elvira Deolinda Rodrigues Pereira Velloso, Carlos Henrique Ares Silveira da Motta, Juliana Braga Furtado, Nydia Strachman Bacal, Paulo Augusto Achucarro Silveira, Cynthia Bachir Moyses, [...]

DOI: 10.1590/S1679-45082011AO2041

ABSTRACT Objective: To study the frequency of mutations that may lead to a good or bad prognosis, as well as their relation with the karyotype and immunophenotype in patients with acute myeloid leukemia. Methods: Thirty samples of patients with acute myeloid leukemia were studied, in which FLT3-ITD, FLT3-TKD and NPM1 mutations were investigated. All samples were submitted to immunophenotyping and 25 to karyotyping. Results: An occurrence of 33.3% NPM1 mutation and an equal number of FLT3-ITD mutation were observed. When […]