All publications of “Ozires Pereira Santos Ramos”

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SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital

Roberta Sitnik, Alexandre Rodrigues Marra, Roberta Cardoso Petroni, Ozires Pereira Santos Ramos, Marinês Dalla Valle Martino, Jacyr Pasternak, [...]

DOI: 10.1590/S1679-45082014AO2932

ObjectiveTo test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.MethodsA total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.ResultsMore positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only […]