06/May/2024
Assessing the toxicity of one-step-synthesized PEG-coated gold nanoparticles: in vitro and in vivo studies
DOI: 10.31744/einstein_journal/2024AO0764
Highlights One-step synthesis provides affordable, stable, and biocompatible nanoparticles. PEG-coated gold nanoparticles exhibited very low cytotoxicity effects. This in vivo study did not reveal hematopoietic, renal, or hepatic alterations. The histopathological analysis did not present any tissue or cellular damage. ABSTRACT Objective: To evaluate the in vitro and in vivo toxicities of polyethylene glycol-coated gold nanoparticles synthesized using a one-step process. Methods: Gold nanoparticles were prepared via a co-precipitation method using polyethylene glycol, and the synthesis product was characterized. For […]
Keywords: Flow cytometry; In vitro techniques; Nanomedicine; Nanoparticles; polyethylene glycols; Toxicity
19/Dec/2022
Immunophenotypic characterization of acute leukemias in Bahia, Brazil
DOI: 10.31744/einstein_journal/2023AO0117
Highlights The incidence of acute myeloid leukemia was slightly higher in women. The most frequent aberrant marker in B-cell ALL was CD66c, followed by CD13 and CD33. Expression of CD34, HLA-DR, CD13, MPO, CD15, CD64, CD2 and CD7 was different in the non-APL and APL AML groups. Differences in expression of CD10, CD13, CD33, CD66c and CD34 were observed in different age groups with B-cell ALL. ABSTRACT Objective To characterize the immunophenotypic profile of acute leukemias in the population […]
Keywords: Acute myeloid leukemia; Flow cytometry; Immunophenotyping; Leukemia; Leukemia, myeloid, acute; Precursor B-cell lymphoblastic leukemia-lymphoma
09/Oct/2020
Comparison of senescence progression in mesenchymal cells from human umbilical cord walls measured by immunofluorescence and flow cytometry of p16 and p21
einstein (São Paulo). 09/Oct/2020;18:eAO5236.
View Article09/Oct/2020
Comparison of senescence progression in mesenchymal cells from human umbilical cord walls measured by immunofluorescence and flow cytometry of p16 and p21
DOI: 10.31744/einstein_journal/2020AO5236
ABSTRACT Objective To follow the expansion of mesenchymal stem cells from umbilical cords by two classic senescence markers, p16 (INK4A) and p21 (CDKN1A), using practical, fast, and less expensive methods than the gold standard Western blotting technique, to evaluate its applicability in the laboratory. Methods Mesenchymal stem cells from umbilical cords were isolated from Wharton’s jelly and, after quality control, morphological and immunophenotypic characterization by flow cytometry, were expanded in culture until coming close to cell cycle arrest (replicative senescence). […]
Keywords: Cellular senescence; Cyclin-dependent kinase inhibitor p21; Flow cytometry; Immunofluorescence; Mesenchymal stem cells
23/Jan/2020
Myelodysplastic syndrome: validation of flow cytometry multilineage score system
DOI: 10.31744/einstein_journal/2020AO4966
ABSTRACT Objective To validate multilineage score system correlating results of flow cytometry, cytogenetics, cytomorphology and histology from samples of patients with suspected myelodysplastic syndrome or cytopenia of unknown origin. Methods A retrospective study analyzing laboratory data of 49 patients with suspected myelodysplastic syndrome or cytopenia of unknown origin, carried out between May and September 2017. The inclusion criteria were availability of flow cytometry results, and at least one more method, such as morphology, histology or cytogenetics. Thirty-eight patients were classified […]
Keywords: Cytogenetics; Cytomorphology; Flow cytometry; Histology; Multilineage score system; Myelodysplastic syndromes
01/Apr/2011
Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies
DOI: 10.1590/S1679-45082011AO1846
ABSTRACT Objective: To identify antiplatelet antibodies by flow cytometry (direct method) in patients with thrombocytopenia. Methods: Between January 1997 and March 2004 a total of 15100 patients were referred to the Centro de Hematologia de São Paulo for hematological investigation of several diagnoses (anemia, leukopenia, thrombocytopenia, coagulation abnormalities, adenomegaly, leukemia and others). Of those, 1057 were referred because of thrombocytopenia and were divided into two groups: Group Idiopathic thrombocytopenic purpura, with no identifiable cause; and Group Other thrombocytopenia, which included […]
Keywords: Blood platelets; Flow cytometry
01/Apr/2011
Correlation between flow cytometry and histologic findings: ten year experience in the investigation of lymphoproliferative diseases
einstein (São Paulo). 01/Apr/2011;9(2 Pt 1):151-9.
View Article01/Apr/2011
Correlation between flow cytometry and histologic findings: ten year experience in the investigation of lymphoproliferative diseases
DOI: 10.1590/S1679-45082011AO2027
ABSTRACT Objective: To demonstrate the advantages of correlating flow cytometry immunophenotyping with the pathology/ immunohistochemistry of lymph nodes or nodules in the diagnosis of lymphoproliferative diseases. Methods: A retrospective study was carried out of 157 biopsy or fine-needle aspiration lymph nodes/ nodule specimens taken from 142 patients, from 1999 and 2009. The specimens were simultaneously studied with fow cytometry and pathology at Hospital Israelita Albert Einstein. The specimens were prepared in hematoxylin/eosin, Giemsa, or monoclonal antibody stained slides for detecting […]
Keywords: Flow cytometry; Immunohistochemistry; Immunophenotyping; Lymphoma; Lymphoproliferative disorders/diagnosis