All publications of “Anna Carla Goldberg”

8 results

Scientific integrity 2.0: misconduct. Let’s prevent, not punish!

Anna Carla Goldberg ORCID logo , Oscar Fernando Pavão dos Santos ORCID logo , Celso Moura Rebello ORCID logo , Jacyr Pasternak ORCID logo

DOI: 10.31744/einstein_journal/2019ED5064

The growing complexity of modern, innovative research has brought up important issues concerning research compliance and responsible conduct of research. The scope of items to be managed have expanded to include a great variety of issues, ranging from authorship, plagiarism, data management, confidentiality, patent rights, conflicts of interest to ethical conduct, animal well-being and social aspects of ongoing research.() All these factors have increasingly burdened the daily routine of wet laboratories, clinical research centers, and animal facilities involved in scientific […]

Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns

Alberto de Castro Pochini, Eliane Antonioli, Daniella Zanetti Bucci, Luiz Roberto Sardinha, Carlos Vicente Andreoli, Mario Ferretti, [...]

DOI: 10.1590/S1679-45082016AO3548

ABSTRACTObjective:To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods.Methods:Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth […]

MHC structure and function − antigen presentation. Part 2

Anna Carla Goldberg, Luiz Vicente Rizzo

DOI: 10.1590/S1679-45082015RB3123

The second part of this review deals with the molecules and processes involved in the processing and presentation of the antigenic fragments to the T-cell receptor. Though the nature of the antigens presented varies, the most significant class of antigens is proteins, processed within the cell to be then recognized in the form of peptides, a mechanism that confers an extraordinary degree of precision to this mode of immune response. The efficiency and accuracy of this system is also the […]

MHC structure and function – antigen presentation. Part 1

Anna Carla Goldberg, Luiz Vicente Rizzo

DOI: 10.1590/S1679-45082015RB3122

The setting for the occurrence of an immune response is that of the need to cope with a vast array of different antigens from both pathogenic and non-pathogenic sources. When the first barriers against infection and innate defense fail, adaptive immune response enters the stage for recognition of the antigens by means of extremely variable molecules, namely immunoglobulins and T-cell receptors. The latter recognize the antigen exposed on cell surfaces, in the form of peptides presented by the HLA molecule. […]

Application of the enterprise management tools Lean Six Sigma and PMBOK in developing a program of research management

Cora Hors, Anna Carla Goldberg, Ederson Haroldo Pereira de Almeida, Fernando Galan Babio Júnior, Luiz Vicente Rizzo

DOI: 10.1590/S1679-45082012000400015

Objective:Introduce a program for the management of scientific research in a General Hospital employing the business management tools Lean Six Sigma and PMBOK for project management in this area.METHODS:The Lean Six Sigma methodology was used to improve the management of the institution’s scientific research through a specific tool (DMAIC) for identification, implementation and posterior analysis based on PMBOK practices of the solutions found.RESULTS:We present our solutions for the management of institutional research projects at the Sociedade Beneficente Israelita Brasileira Albert […]

Isolation and characterization of mesenchymal stem cells obtained from reusable and disposable bone marrow collection filters

Glaziane Cordeiro de Deus, Marilia Normanton, Nelson Hamerschlak, Andrea Tiemi Kondo, Andreza Alice Feitosa Ribeiro, Anna Carla Goldberg, [...]

DOI: 10.1590/S1679-45082012000300007

Objective:To compare human mesenchymal stem cells obtained from reusable and disposable filters and to characterize them according to the criteria of the International Society of Cellular Therapy.METHODS:Human mesenchymal stem cells were isolated from bone marrow collection reusable sets and compared with those obtained from disposable sets by washing the filters with cell culture media. The isolated cells were characterized according to the criteria of the International Society of Cellular Therapy using flow cytometry, differentiation in vitro, and cytochemistry techniques.RESULTS:Samples were […]

Frequency of single nucleotide polymorphisms of some immune response genes in a population sample from São Paulo, Brazil

Léa Campos de Oliveira, Rajendranath Ramasawmy, Jaila Dias Borges, Maria Lucia Carnevale Marin, Natalie Guida Muller, Jorge Kalil, [...]

DOI: 10.1590/s1679-45082011ao1866

ABSTRACTObjective:To present the frequency of single nucleotide polymorphisms of a few immune response genes in a population sample from São Paulo City (SP), Brazil.Methods:Data on allele frequencies of known polymorphisms of innate and acquired immunity genes were presented, the majority with proven impact on gene function. Data were gathered from a sample of healthy individuals, non-HLA identical siblings of bone marrow transplant recipients from the Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, obtained between 1998 […]

Contrasting roles of donor and recipient TGFB1 and IFNG gene polymorphic variants in chronic kidney transplant rejection

Verônica Porto Carreiro de Vasconcellos Coelho, Rafael Ioschpe, Cristina Caldas, Monica Spadafora-Ferreira, João Americo Fonseca, Maria Regina Alves Cardoso, [...]

DOI: 10.1590/S1679-45082011AO1852

ABSTRACT Objective: To assess the long-term impact (minimum of 3 years follow-up) of polymorphisms in cytokine genes in donor:recipient pairs on the results of the transplant. Methods: We compared genetic cytokine polymorphisms and the primary factors of risk for the development of chronic rejection in paired groups of renal transplant patients with and without chronic allograft nephropathy [CAN]. Results: Multivariate analysis indicated that the presence of the high-production TT genotype (codon 10) of the transforming growth factor beta-1 (TGFB1) was […]