2 results

Isolation and characterization of mesenchymal stem cells obtained from reusable and disposable bone marrow collection filters

Glaziane Cordeiro de Deus, Marilia Normanton, Nelson Hamerschlak, Andrea Tiemi Kondo, Andreza Alice Feitosa Ribeiro, Anna Carla Goldberg, [...]

DOI: 10.1590/S1679-45082012000300007

Objective:To compare human mesenchymal stem cells obtained from reusable and disposable filters and to characterize them according to the criteria of the International Society of Cellular Therapy.METHODS:Human mesenchymal stem cells were isolated from bone marrow collection reusable sets and compared with those obtained from disposable sets by washing the filters with cell culture media. The isolated cells were characterized according to the criteria of the International Society of Cellular Therapy using flow cytometry, differentiation in vitro, and cytochemistry techniques.RESULTS:Samples were […]

Sterilization of single-use helical stone baskets: an experimental study

Fernando Korkes, Alex Menezes, Cely Barreto da Silva, Roni de Carvalho Fernandes, Marjo Deninson Cardenuto Perez

DOI: 10.1590/S1679-45082011AO1762

ABSTRACT Objectives: To experimentally evaluate the efficacy of a standard sterilization protocol employed during reuse of disposable helical stone baskets. Methods: Study performed on 20 helical stone baskets: 10 were used in the initial validation process, contaminated with Escherichia coli ATCC 25922 and imprinted on Müeller-Hinton media; 10 catheters were contaminated with Geobacillus stearothermophilus ATCC 7953, processed, inoculated in TSB and incubated in a water bath at a temperature of 55°C. Bacterial growth was evaluated after 1, 3, 5 and […]