einstein (São Paulo). 23/Jun/2023;21:eAE0115.
RT-qPCR-based pool testing for the diagnosis of COVID-19
Hugo Itaru , Murilo Soares , Ricardo Hiroshi Caldeira , Karine Lima , Nathalia Sernizon , Claudia Regina Lindgren , Elaine Leandro , Unaí , Flávio Guimarães da , Santuza Maria Ribeiro
DOI: 10.31744/einstein_journal/2023AE0115
Highlights
Pool testing is simultaneously performed on multiple samples to efficiently and cost-effectively detect COVID-19.
Pool testing can optimize resource utilization and expand diagnostic access, and is a viable alternative for developing countries with limited access to testing.
To optimize resources, the pool size was determined by estimating COVID-19 prevalence in the study population.
ABSTRACT
This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed by quantitative real-time polymerase chain reaction using pool testing to detect SARS-CoV-2 in nasopharyngeal swab samples. The study proposes an innovative diagnostic strategy that contributes to resource optimization, cost reduction, and improved agility of feedback from results.
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RT-qPCR-based pool testing for the diagnosis of COVID-19
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