Category: Respiratory Endoscopy Introduction: Histopathological analyses and classification by the Tumor, Node, Metastasis System (TNM) are key-elements in therapeutic decision-making for non-small cell lung cancer (NSCLC).() Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is effective in obtaining biopsies of hilar and mediastinal lymph nodes (MLN), for tissue collection to mediastinal staging in NSCLC.(,) The assessment of clinically significant genomic alterations in surgically resected primary tumors (PT) and MLN aspirates obtained by EBUS-TBNA can provide valuable insights into the risk of recurrence, […]

Category: Thoracic Oncology Introduction: Genomic mutational profiling, obtained through next-generation sequencing (NGS) plays a pivotal role in assisting clinicians in therapeutic decision- making in patients with non-small cell cancer (NSCLC).(,) However, these techniques require high quality and sufficient quantity of material to produce reliable results.(,) Our study proposes the use of tissue scraping from histological and cytological slides as an alternative method for obtaining genomic DNA (gDNA) of better quality and quantity, and also an optimized extraction protocol from paraffin […]