1 results
22/Jan/2020
22/Jan/2020
DOI: 10.31744/einstein_journal/2020AO5132
ABSTRACT Objective To evaluate the performance of enzyme-linked immunosorbent assay and indirect immunofluorescence methods for the detection of antineutrophil cytoplasmic antibodies in a routine clinical laboratory setting. Methods A total of 227 samples were tested by indirect immunofluorescence and enzyme-linked immunosorbent assay with antigen specificity for antiproteinase 3 and antimyeloperoxidase. The proportions of positive samples were compared by McNemar hypotheses and agreement was described by Cohen’s Kappa coefficient. Results The agreement of the tests was 96.5%, and the Kappa coefficient […]
Keywords: Antibodies, antineutrophil cytoplasmic; Autoantibodies; Autoimmune diseases; Enzyme-linked immunosorbent assay; Fluorescent antibody technique, indirect