Clinical metagenomic sequencing for diagnosis of meningitis and encephalitis
Methagenomic next generation sequencing of cerebrospinal fluid proved to be able to diagnose the etiology of meningitis and encephalitis in 204 hospitalized patients with these problems. The methodology could diagnose 58 infections in 57 patients. Among those 58 infections metagenomic sequencing, researchers were able to diagnose 13 that were not diagnosed by usual clinical testing. Among the remaining, 45 infections metagenomic sequencing had the same results of other laboratory tests in 19 cases. Of the 26 infections not diagnosed by metagenomic sequencing 11 were diagnosed by serological testing, and 7 diagnosed by histological testing. The 8 cases diagnosed by metagenomic testing only had clinical impact considering that efficient therapies are viable for 7 of them.
Wilson MR, Sample HA, Zorn KC, Arevalo S, Yu G, Neuhaus J, et al. Clinical metagenomic sequencing for diagnosis of meningitis and encephalitis. N Engl J Med. 2019;380(24):2327-40. https://doi.org/10.1056/NEJMoa1803396.