1 results
25/Jun/2024
25/Jun/2024
DOI: 10.31744/einstein_journal/2024ABS_BTS_STO006
Category: Thoracic Oncology Introduction: Genomic mutational profiling, obtained through next-generation sequencing (NGS) plays a pivotal role in assisting clinicians in therapeutic decision- making in patients with non-small cell cancer (NSCLC).(,) However, these techniques require high quality and sufficient quantity of material to produce reliable results.(,) Our study proposes the use of tissue scraping from histological and cytological slides as an alternative method for obtaining genomic DNA (gDNA) of better quality and quantity, and also an optimized extraction protocol from paraffin […]
Keywords: EBUS-TBNA; FFPE material; gDNA extractione; Mediastinal lymph node; Scraped slides