einstein (São Paulo). 05/set/2022;20:eCE0018.
B-cell precursors (hematogones) in CD34+ hematopoietic stem cell collections: a single-center experience
DOI: 10.31744/einstein_journal/2022CE0018
So, where do we stand?
At our center we objectively draw attention to the presence of B-cell precursors in flow cytometry reports whenever the total number of CD34+ HSC, excluding the presence of B-cell precursors, is less than 2.0 x 106 cells per kilogram of recipient’s weight. Therefore, to alert the transplant physician about the possibility that – due to the presence of B-cell precursors – the bag destined for the HSCT may contain a suboptimal number of CD34+ HSC, we suggest the laboratories three simple actions as general recommendation.
First, for the quantification of B-cell precursors, the addition of an anti-CD19 antibody () in the standard tube (anti-CD45, anti-CD34, 7-AAD) to increase the precision of quantification, at least for some cases where the delimitation of small B-cell precursor populations, based only on physical properties (forward scatter and side scatter) and low intensity CD45 expression, can be difficult and less accurate. Second, the percentage of B-cell precursors and the number of CD34+ HPC non-B-cell precursor cells per kilogram of recipient’s weight in the bag should be reported. Third, a detailed report, preferably including some brief considerations about what the presence of B-cell precursors means, could help the transplant physician make better clinical decisions. In our center, we systematically include in our flow cytometry report, the following advice, as a final note:
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