Volume 9, Number 4, October/December 2011

Liver markers, prevalence of the metabolic syndrome abnormalities and effect of Roux-en-Y gastric bypass in morbidly obese subjects

Ary Serpa Neto, Felipe Martin Bianco Rossi, Rodrigo Dal Moro Amarante, Marçal Rossi

DOI: 10.1590/s1679-45082011ao2042

ABSTRACTObjectives:To evaluate the relations between liver markers (GGT, ALT and AST) and the metabolic syndrome (and its components) in morbidly obese subjects, and to determine the response of these metabolic factors and hepatic enzymes after weight loss induced by Roux-en-Y gastric bypass.Methods:This study was carried out at a university hospital, in Santo André (SP), Brazil. We evaluated 140 morbidly obese subjects aged from 18 to 60 years submitted to a Roux-en-Y gastric bypass, who were followed for a mean period […]

Nanostructured SBA-15 silica as an adjuvant in immunizations with hepatitis B vaccine

Karina Scaramuzzi, Denise Cristina André Oliveira, Luciana Vieira Carvalho, Denise Vilarinho Tambourgi, Elisabeth Christina Nunes Tenório, Marisa Rizzi, [...]

DOI: 10.1590/s1679-45082011ao2162

ABSTRACTObjective:To evaluate the applicability of SBA-15 silica as an adjuvant in immunizations with purified particles of the viral protein HBsAg, the main component of hepatitis B vaccine, Butang®, produced by Instituto Butantan.Methods:BALB/c mice orally or subcutaneously received 0.5 μg of HBsAg adsorbed/encapsulated to SBA-15 or adsorbed to Al(OH)3. To assess the secondary immune response, a subcutaneous booster was administered 30 days after the first immunization. Individual serum and fecal samples of each group were periodically collected for specific antibody titration […]

Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes

Flavia Maziero Andreghetto, Maria Fatima Guarizo Klingbeil, Renata Machado Soares, Roberta Sitnik, Décio dos Santos Pinto Junior, Monica Beatriz Mathor, [...]

DOI: 10.1590/s1679-45082011ao2149

ABSTRACTObjective:Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas.Methods:The expression level of four microRNAs was assessed in cell lines and in primary cultures of […]

Isolation of human umbilical cord blood-derived osteoprogenitor cells: a promising candidate for cell-based therapy for bone repair

Igor Iuco Castro-Silva, Letícia de Oliveira Castro, Janaína José dos Santos Machado, Maria Helena Alves Nicola, José Mauro Granjeiro

DOI: 10.1590/s1679-45082011ao2196

ABSTRACTObjective:The aim of this study was to evaluate the osteogenic potential of human umbilical cord blood-derived osteoprogenitor cells and to prove its applicability as a promising candidate for cell-based therapeutics for bone repair.Methods:Primary cultures of human umbilical blood cord adherent cells were expanded in vitro until passage 2 and seeded for osteodifferentiation study. Morphological (light microscopy), cytochemical (Von Kossa’s method), and functional analyses (calcium level, alkaline phosphatase activity, and total protein content in cell culture) were carried out 7, 14, […]

Implementation and progress of clinical pharmacy in the rational medication use in a large tertiary hospital

Fábio Teixeira Ferracini, Silvana Maria de Almeida, Juliana Locatelli, Sandra Petriccione, Celina Setsuko Haga

DOI: 10.1590/s1679-45082011ao2140

ABSTRACTObjective:To show developments and contributions of a clinical pharmacy service in safety and rational use of medications in a large tertiary hospital.Methods:Clinical pharmacists were responsible for all issues using medications at the hospital. In the beginning this professional was responsible for analyzing medical prescriptions, horizontal visits, and to set up protocols. Afterwards, other activities were designated such as monitoring for drug safety, participating in committees and managed routines. If problems were found, the pharmacist reported them for the physician and, […]

Incidence of sleep disorders in patients with Alzheimer disease

Einstein Francisco Camargos, Marcela Basso Pandolfi, Marco Polo Dias, Juliana Lima Quintas, Renato Maia Guimarães, Otávio de Toledo Nóbrega

DOI: 10.1590/s1679-45082011ao2145

ABSTRACTObjective:To determine the incidence of sleep disorder at a follow-up examination from 1 to 4 years, in demented patients diagnosed at first visit, besides analyzing associated demographic and comorbidities characteristics.Methods:A total of 122 elderly patients aged 60 years or older and diagnosed with dementia (Alzheimer and other) were followed in a reference geriatric center for dementia. The clinical protocols included interviews with patient and caregiver, complete physical examination, laboratory and imaging tests. Criteria for the diagnosis of sleep disorder included […]

Vertigo and dizziness in elderly patients with neurological disorders

Pedro Luiz Mangabeira Albernaz, Flávia Silveira dos Santos Cabral

DOI: 10.1590/s1679-45082011ao2131

ABSTRACTObjective:To evaluate the frequency of central vestibular disorders, vertigo and dizziness in patients aged 65 to 90 years.Methods:We analyzed 735 patients in this age range seen consecutively from January 1990 to December 2009.Results:A total of 32 patients had central vestibular disorders, and 11 had neurological disorders diagnosed by imaging.Conclusion:Vertigo and dizziness occur frequently in elderly people with neurological disorders, and affect their quality of life. Falling is common and may lead to disabling consequences.

Postural control in blind subjects

Antonio Vinicius Soares, Cláudia Silva Remor de Oliveira, Rodrigo José Knabben, Susana Cristina Domenech, Noe Gomes Borges Junior

DOI: 10.1590/s1679-45082011ao2046

ABSTRACTObjective:To analyze postural control in acquired and congenitally blind adults.Methods:A total of 40 visually impaired adults participated in the research, divided into 2 groups, 20 with acquired blindness and 20 with congenital blindness − 21 males and 19 females, mean age 35.8 ± 10.8. The Brazilian version of Berg Balance Scale and the motor domain of functional independence measure were utilized.Results:On Berg Balance Scale the mean for acquired blindness was 54.0 ± 2.4 and 54.4 ± 2.5 for congenitally blind […]

Quality assessment of inhospital patients unable to speak who use alternative and extended communication

Fabrício Marinho Bandeira, Flávia Perassa de Faria, Everaldo Batista de Araujo

DOI: 10.1590/s1679-45082011ao2083

ABSTRACTObjective:To implement the use of alternative communication boards in patients hospitalized and unable to communicate by speech.Methods:A descriptive study with a sample of 30 patients hospitalized and unable to communicate by speech. Boards were made with Boardmaker® Software that consists of a graphical database containing Pictorial Communication Symbols. To assess the quality of life of these patients, the Portuguese SF-36, a translated version of the Medical Outcomes Study, was applied before and after the study.Results:We found that all domains of […]

Clinical and epidemiological aspects of prematurity in a Normal Delivery Center, São Paulo, Brazil

Miriam Siqueira do Carmo Rabello, Sonia Maria Oliveira de Barros

DOI: 10.1590/s1679-45082011ao1874

ABSTRACTObjective:To present clinical and epidemiological aspects of prematurity in a Normal Delivery Center, and to describe outcomes.Methods:A cross-sectional retrospective study, with 189 medical records of premature, single, and living fetuses, with gestational ages between 25 and 36.6 weeks.Results:The prevalence of preterm births was 7.8%, and 23.2% in adolescent mothers. The most frequent obstetric complication was premature rupture of ovular membranes (35.4%), followed by hypertensive syndrome (18.5%). Cesarean section was performed in 33% of cases. The Apgar score in the 1st […]

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