All publications of “Paulo Henrique Silveira”

2 results

Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line

Javier Bustamante Mamani, Lorena Favaro Pavon, Liza Aya Mabuchi Miyaki, Tatiana Tais Sibov, Fabiana Rossan, Paulo Henrique Silveira, [...]

DOI: 10.1590/S1679-45082012000200016

Objective:To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications.METHODS:Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification […]

In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles

Javier Bustamante Mamani, Jackeline Moraes Malheiros, Ellison Fernando Cardoso, Alberto Tannús, Paulo Henrique Silveira, Lionel Fernel Gamarra

DOI: 10.1590/S1679-45082012000200009

Objective:The aim of the current study was to monitor the migration of superparamagnetic iron oxide nanoparticle (SPION)-labeled C6 cells, which were used to induce glioblastoma tumor growth in an animal model, over time using magnetic resonance imaging (MRI), with the goal of aiding in tumor prognosis and therapy.METHODS:Two groups of male Wistar rats were used for the tumor induction model. In the first group (n=3), the tumors were induced via the injection of SPION-labeled C6 cells. In the second group […]