All publications of “Patricia Severino”

2 results

Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes

Flavia Maziero Andreghetto, Maria Fatima Guarizo Klingbeil, Renata Machado Soares, Roberta Sitnik, Décio dos Santos Pinto Junior, Monica Beatriz Mathor, [...]

DOI: 10.1590/s1679-45082011ao2149

ABSTRACTObjective:Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas.Methods:The expression level of four microRNAs was assessed in cell lines and in primary cultures of […]

Biomarkers in Parkinson Disease: global gene expression analysis in peripheral blood from patients with and without mutations in PARK2 and PARK8

Patricia Maria de Carvalho Aguiar, Patricia Severino

DOI: 10.1590/s1679-45082010ao1674

ABSTRACTObjective:To evaluate the performance of gene expression analysis in the peripheral blood of Parkinson disease patients with different genetic profiles using microarray as a tool to identify possible diseases related biomarkers which could contribute to the elucidation of the pathological process, as well as be useful in diagnosis.Methods:Global gene expression analysis by means of DNA microarrays was performed in peripheral blood of Parkinson disease patients with previously identified mutations in PARK2 or PARK8 genes, Parkinson disease patients without known mutations […]