All publications of “Fernando Beani Margeotto”

1 results

Glycomics expression analysis of sulfated glycosaminoglycans of human colorectal cancer tissues and non-neoplastic mucosa by electrospray ionization mass spectrometry

Ana Paula Cleto Marolla, Jaques Waisberg, Gabriela Tognini Saba, Daniel Reis Waisberg, Fernando Beani Margeotto, Maria Aparecida da Silva Pinhal

DOI: 10.1590/S1679-45082015AO3477

ABSTRACTObjective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression.Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans […]