All publications of “Maria Aparecida da Silva Pinhal”

3 results

Inflammatory biomarkers in sera of patients with intervertebral disc degeneration

Luciano Miller Reis Rodrigues ORCID logo , Lilian Zerbinatti de Oliveira ORCID logo , Mariane de Barros Ribeiro da Silva ORCID logo , Camila de Melo Accardo ORCID logo , Adriana Braz Del Giglio ORCID logo , Maria Aparecida da Silva Pinhal ORCID logo

DOI: 10.31744/einstein_journal/2019AO4637

ABSTRACT Objective: To evaluate intervertebral disc levels of inflammatory factor (interleukin 6) and proteinase activity (cathepsin B) in patients with a degenerative disease and serum levels of interleukin 6, serum cathepsin B activity and hyaluronic acid biomarkers. Methods: We conducted immunohistochemistry studies of intervertebral discs to analyze interleukin 6 and cathepsin B levels of patients with degenerative disease and spine fracture (Control Group) and to measure hyaluronic acid, interleukin 6 and cathepsin B activity from sera of intervertebral disc degeneration […]

Glycomics expression analysis of sulfated glycosaminoglycans of human colorectal cancer tissues and non-neoplastic mucosa by electrospray ionization mass spectrometry

Ana Paula Cleto Marolla, Jaques Waisberg, Gabriela Tognini Saba, Daniel Reis Waisberg, Fernando Beani Margeotto, Maria Aparecida da Silva Pinhal

DOI: 10.1590/S1679-45082015AO3477

ABSTRACTObjective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression.Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans […]

Cells involved in extracellular matrix remodeling after acute myocardial infarction

Larissa Ferraz Garcia, Fábio D’Aguiar Mataveli, Ana Maria Amaral Antônio Mader, Thérèse Rachell Theodoro, Giselle Zenker Justo, Maria Aparecida da Silva Pinhal

DOI: 10.1590/S1679-45082015AO2970

ObjectiveEvaluate the effects of VEGF165 gene transfer in the process of remodeling of the extracellular matrix after an acute myocardial infarct.MethodsWistar rats were submitted to myocardial infarction, after the ligation of the left descending artery, and the left ventricle ejection fraction was used to classify the infarcts into large and small. The animals were divided into groups of ten, according to the size of infarcted area (large or small), and received or not VEGF165 treatment. Evaluation of different markers was […]